|
|
|
| 109 - THIOBACILLUS FERROOXIDANS MEDIUM (APH) | ||
| Distilled water | 1.0 | l |
| FeSO4 x 7 H2O | 40.0 | g |
| Ca(NO3)2 | 0.01 | g |
| KCl | 0.1 | g |
| MgSO4 x 7 H2O | 0.5 | g |
| K2HPO4 | 0.5 | g |
| (NH4)2SO4 | 2.0 | g |
| Adjust pH to 3.0 with dilute H2SO4 or KOH. Sterilise the medium by filtration. Alternatively, autoclave separately the basal medium (pH adjusted to 3.0) and the ferrous sulphate (40 g FeSO4 x 7 H2O in 100 ml distilled water, pH not adjusted, in a sealed vessel under nitrogen gas atmosphere). | ||
|
|