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> Media 135
135 - MINERAL MEDIUM WITH TOLUENE
Distilled water
1.0
l
Nitrilotriacetic acid (NTA)
10.0
mg
CoSO
4
10.0
µg
ZnSO
4
x 7 H
2
O
0.2
mg
MnSO
4
x H
2
O
3.0
mg
Ferric ammonium citrate
5.0
mg
MgSO
4
x 7 H
2
O
0.2
g
NH
4
Cl
2.0
g
KH
2
PO
4
1.5
g
Na
2
HPO
4
x 12 H
2
O
9.0
g
Adjust pH to 7.0. One drop of toluene is evaporated in a desiccator. 0.1% succinate may be added to the medium as additional carbon source.
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