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Media
> Media 140
140 - M63 MEDIUM
Solution C
1
ml
Solution B
20
ml
Sterile water
779
ml
Solution A
200
ml
Mix solution A with sterile water. Add solutions B and C. Mix well and aseptically prepare plates, tubes or bottles.
Solution A
pH 7.0
Distilled water to
1.0
l
FeSO
4
x 7 H
2
O
2.5
g
(NH
4
)
2
SO
4
10.0
g
KH
2
PO
4
68.0
g
Dissolve the components to water by heating. Autoclave 121°C for 15 min.
Solution B
Distilled water to
200.0
ml
Casamino acids
50.0
g
Dissolve amino acids to water by heating and filter-sterilize.
Solution C
Distilled water to
100.0
ml
MgSO
4
x 7 H
2
O
24.65
g
Mix carefully and filter-sterilize.
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