The vitamin K1, haemin solution and cysteine are added after
the medium has been boiled and cooled under CO2. Adjust pH to
7.2 using 8 N NaOH. Distribute under N2 and autoclave.
Haemin solution
Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled
water. Store refrigerated.
Vitamin solution
Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter
sterilize. Store refrigerated in a brown bottle for max. one month.
