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> Media 68
68 - YEAST SYNTHETIC COMPLETE MEDIUM (SC)
*Synthetic stock solution
Distilled water
300.0
ml
Valine
1.17
g
Uracil
0.22
g
Tyrosine
0.18
g
Tryptophan
0.82
g
Threonine
1.19
g
Serine
1.05
g
Phenylalanine
0.83
g
Methionine
1.49
g
Lysine
0.91
g
Leucine
2.62
g
Isoleucine
5.25
g
Myo-inositol
0.36
g
Histidine
0.58
g
Aspartic acid
2.66
g
Arginine
3.48
g
Adenine
0.135
g
Storage in darkness at room temperature.
Agar
20.0
g
Synthetic stock solution*
30.0
ml
40% (w/v) glucose solution
50.0
ml
Milli-Q water
920.0
ml
Yeast Nitrogen Base w/o amino acids*
6.7
g
*Difco 0919. Glucose and synthetic stock solutions are filter sterilized and added after autoclaving.
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