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>
Media
> Media 93
93 - BIFIDOBACTERIUM MEDIUM
Distilled water
950
ml
Resazurin (25 mg/100 ml)
4
ml
Salt solution (see below)
40
ml
Cysteine-HCl x H
2
O
0.5
g
NaCl
5.0
g
Tween 80
1.0
ml
MnSO
4
x H
2
O
0.05
g
MgSO
4
x 7 H
2
O
0.2
g
K
2
HPO
4
2.0
g
Glucose
10.0
g
Bacto Soytone
5.0
g
Yeast extract
5.0
g
Meat extract
5.0
g
Casein peptone, tryptic digest
10.0
g
The cysteine is added after the medium has been boiled and cooled under CO
2
. Adjust pH to 6.8 using 8 N NaOH. Distribute under N
2
and autoclave.
Salt solution
Distilled water
1.00
l
NaCl
2.00
g
NaHCO
3
10.00
g
KH
2
PO
4
1.00
g
K
2
HPO
4
1.00
g
MgSO
4
x 7 H
2
O
0.50
g
CaCl
2
x 2 H
2
O
0.25
g
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