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104 - 731 LUMINOUS MEDIUM
Distilled water1.0l
Glyserol3.0ml
1 M Tris-buffer pH 7.550.0ml
CaCO31.0g
KCl0.75g
MgSO4 x 7 H2O1.0g
NH4Cl5.0g
KH2HPO42.1g
NaCl30.0g
Yeast extract5.0g
Tryptone5.0g
Autoclave 25 ml in 100 ml erlenmeyers at 121°C for 20 min.
 

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