Online catalogue

106 - EDINBURGH MINIMAL MEDIUM (EMM 2)
***10,000X Minerals
Citric acid10.0g
CuSO4 x 5 H2O0.4g
KI1.0g
Molybdic acid0.4g
FeCl2 x 6 H2O2.0g
ZnSO4 x 7 H2O4.0g
MnSO44.0g
Boric acid5.0g
After autoclaving, a few drops of preservative (1:1:2 chlorobenzene:dichloroethane:chlorobutane) is added to mineral stock.
 
**1000X Vitamins
Distilled water to1.0l
Biotin10.0mg
Inositol10.0g
Nicotinic acid10.0g
Pantothenic acid1.0g
 
 
*50X Salts
Distilled water to1.0l
Na2SO42.0g
KCl50.0g
CaCl2 x 2 H200.735g
MgCl2 x 6 H2052.5g
 
 
Distilled water to1.0l
Minerals (10000X stock)***0.1ml
Vitamins (1000X stock)**1.0ml
Salts (50X stock)*20.0ml
Glucose20.0g
NH4Cl5.0g
Na2HPO42.2g
Potassium hydrogen phthallate (14.7mM)3.0g
 
 

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