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109 - THIOBACILLUS FERROOXIDANS MEDIUM (APH)
Distilled water1.0l
FeSO4 x 7 H2O40.0g
Ca(NO3)20.01g
KCl0.1g
MgSO4 x 7 H2O0.5g
K2HPO40.5g
(NH4)2SO42.0g
Adjust pH to 3.0 with dilute H2SO4 or KOH. Sterilise the medium by filtration. Alternatively, autoclave separately the basal medium (pH adjusted to 3.0) and the ferrous sulphate (40 g FeSO4 x 7 H2O in 100 ml distilled water, pH not adjusted, in a sealed vessel under nitrogen gas atmosphere).
 

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