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> Media 111
111 - THERMUS 162 MEDIUM
**Trace element solution
Distilled water
1.0
l
CoSO
4
0.01
mg
VOSO
4
x 2 H
2
O
0.03
mg
Na
2
MoO
4
x 2 H
2
O
0.03
mg
CuCl
2
x 2 H
2
O
0.05
mg
ZnSO
4
x 7 H
2
O
0.22
mg
Na
2
B
4
O
7
x 10 H
2
O
4.50
mg
MnCl
2
x 4 H
2
O
1.80
mg
*Phosphate buffer
Distilled water
1.0
l
Na
2
HPO
4
x 12 H
2
O
43.0
g
KH
2
PO
4
5.44
g
Adjust pH to 6.5.
Distilled water
900.0
ml
Trace element solution**
100.0
ml
Phosphate buffer*
0.5
ml
0.01 M Fe citrate
0.5
ml
MgCl
2
x 6 H
2
O
200.0
mg
CaSO
4
x 2 H
2
O
40.0
mg
Nitrilotriacetic acid
100.0
mg
Agar
28.0
g
Tryptone
2.5
g
Yeast extract
2.5
g
Adjust pH to 6.5 with NaOH. Autoclave the phosphate buffer separately and then add to the medium.
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