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111 - THERMUS 162 MEDIUM
**Trace element solution
Distilled water1.0l
CoSO40.01mg
VOSO4 x 2 H2O0.03mg
Na2MoO4 x 2 H2O0.03mg
CuCl2 x 2 H2O0.05mg
ZnSO4 x 7 H2O0.22mg
Na2B4O7 x 10 H2O4.50mg
MnCl2 x 4 H2O1.80mg
 
 
*Phosphate buffer
Distilled water1.0l
Na2HPO4 x 12 H2O43.0g
KH2PO45.44g
Adjust pH to 6.5.
 
Distilled water900.0ml
Trace element solution**100.0ml
Phosphate buffer*0.5ml
0.01 M Fe citrate0.5ml
MgCl2 x 6 H2O200.0mg
CaSO4 x 2 H2O40.0mg
Nitrilotriacetic acid100.0mg
Agar28.0g
Tryptone2.5g
Yeast extract2.5g
Adjust pH to 6.5 with NaOH. Autoclave the phosphate buffer separately and then add to the medium.
 

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