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116 - DESULFOBACTER POSTGATE MEDIUM
Solution A is boiled for a few minutes, cooled to room temperature, gassed with 80% N2 + 20% CO2 gas mixture to reach a pH below 6, then autoclaved anaerobically under the same gas mixture. Solutions B, D, E and F are autoclaved separately under nitrogen. Solution C is filter-sterilized and flushed with 80% N2 + 20% CO2 to remove dissolved oxygen. Solution B to F are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anaerobically under 80% N2 + 20% CO2 into appropriate vessels. Final pH of the medium is 7.1 - 7.4. Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth at the beginning. For transfers use 5 - 10% inoculum.
 
Solution A
Distilled water870.0ml
Resazurin1.0mg
CaCl2 x 2 H2O0.15g
KCl0.5g
MgCl2 x 6 H2O1.3g
NaCl7.0g
NH4Cl0.3g
KH2PO40.2g
Na2SO43.0g
 
 
Solution B
Trace element solution SL-101.0ml
 
 
Solution C
Distilled water100.0ml
NaHCO35.0g
 
 
Solution D
Distilled water10.0ml
Na-acetate x 3 H2O2.5g
 
 
Solution E
Vitamin solution10.0ml
 
 
Solution F
Distilled water10.0ml
Na2S x 9 H2O0.4g
 
 
Trace element solution SL-10
Distilled water990.0ml
Na2MoO4 x 2 H2O36.0mg
NiCl2 x 6 H2O24.0mg
CuCl2 x 2 H2O2.0mg
CoCl2 x 6 H2O190.0mg
H3BO36.0mg
MnCl2 x 4 H2O100.0mg
ZnCl270.0mg
FeCl2 x 4 H2O1.5g
HCl (25%; 7.7 M)10.0ml
First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.
 
Vitamin solution
Distilled water1.0l
Lipoic acid5.0mg
p-Aminobenzoic acid5.0mg
Vitamin B120.1mg
D-Ca-pantothenate5.0mg
Nicotinic acid5.0mg
Riboflavin5.0mg
Thiamine-HCl x 2 H2O5.0mg
Pyridoxine-HCl10.0mg
Folic acid2.0mg
Biotin2.0mg
 
 

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