VTT Culture Collection

118 - DESULFOBACTERIUM MEDIUM

Solution A is prepared and autoclaved anaerobically under 80% N₂ + 20% CO₂. Solution C is filter-sterilized and gassed for 20 min with N₂/CO₂. Solutions B, D, E and F are filter-sterilized and gassed with N₂. Solution G is autoclaved under N₂. Solutions B to G are added to the sterile, cooled solution A in the sequence as indicated. Final pH of the medium should be 7.0. Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N₂ and filter-sterilized) may stimulate growth of all strains at the beginning. For transfers use 5 - 10% inoculum. Incubate all strains in the dark.

Solution A
Distilled water	930.0	ml
Resazurin	1.0	mg
CaCl₂ x 2 H₂O	0.15	g
KCl	0.5	g
MgCl₂ x 6 H₂O	3.0	g
NaCl	21.0	g
NH₄Cl	0.3	g
KH₂PO₄	0.2	g
Na₂SO₄	3.0	g


Solution B
Trace elements SL-10 (see medium 116)	1.0	ml


Solution C
Distilled water	50.0	ml
NaHCO₃	2.5	g


Solution D

Substrate (see below)

Solution D for E-001657
Distilled water	10.0	ml
Na-acetate x 3 H₂O	2.5	g


Solution D for E-001658

Use a gas phase of 80% H₂ + 20% CO₂ (= substrate). Cells do not tolerate temperatures higher than 28°C.

Solution E
Vitamin solution (see medium 116)	1.0	ml


Solution F
Na₂SeO₃ x 5 H₂O -solution*	1.0	ml
*3 mg in 1 l of 0.01 M NaOH

Solution G
Distilled water	10.0	ml
Na₂S x 9 H₂O	0.4	g
Solution A is prepared and autoclaved anaerobically under 80% N2 + 20% CO2. Solution C is filter-sterilized and gassed for 20 min with N2/CO2. Solutions B, D, E and F are filter-sterilized and gassed with N2. Solution G is autoclaved under N2. Solutions B to G are added to the sterile, cooled solution A in the sequence as indicated. Final pH of the medium should be 7.0. Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of all strains at the beginning. For transfers use 5 - 10% inoculum. Incubate all strains in the dark.

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