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> Media 121
121 - Mineral Sals Medium with Octane
Agar, if needed
15.0
g
Tap water
1.0
l
Na
2
SO
4
0.5
g
K
2
HPO
4
5.0
g
(NH
4
)
2
HPO
4
10.0
g
Autoclave at 121ºC for 15 min (solid medium) or filter sterilize (broth). Supplement this mineral medium with filter-sterilized octane (1% v/v) at the time of use.
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