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121 - Mineral Sals Medium with Octane
Agar, if needed15.0g
Tap water1.0l
Na2SO40.5g
K2HPO45.0g
(NH4)2HPO410.0g
Autoclave at 121ºC for 15 min (solid medium) or filter sterilize (broth). Supplement this mineral medium with filter-sterilized octane (1% v/v) at the time of use.
 

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