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Media
> Media 124
124 - THERMOANAEROBIUM MEDIUM
Distilled water
1.0
l
Glucose
5.0
g
Tryptone or Trypticase
10.0
g
Yeast extract
3.0
g
Na
2
S x 9 H
2
O
1.0
g
Vitamin solution (see medium 116)
5.0
ml
Resazurin
1.0
mg
FeSO
4
x 7 H
2
O
3.0
mg
Trace element solution (see below)
9.0
ml
K
2
HPO
4
1.5
g
KH
2
PO
4
0.75
g
MgCl
2
x 6 H
2
O
0.4
g
NaCl
0.9
g
NH
4
Cl
0.9
g
The medium is prepared under 100% N
2
gas. Glucose is sterilized separately. Final pH of the medium is 7.2 - 7.4.
Trace element solution
Distilled water
1.0
l
Na
2
SeO
3
x 5 H
2
O
0.02
g
NaCl
1.0
g
NiCl
2
x 6 H
2
O
0.026
g
Na
2
MoO
4
x 2 H
2
O
0.01
g
H
3
BO
3
0.01
g
CuCl
2
0.02
g
ZnCl
2
0.1
g
CaCl
2
x 2 H
2
O
0.1
g
CoCl
2
x 6 H
2
O
0.17
g
MnCl
2
x 4 H
2
O
0.1
g
FeCl
2
x 4 H
2
O
0.2
g
Nitrilotriacetic acid
12.8
g
First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH.
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