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124 - THERMOANAEROBIUM MEDIUM
Distilled water1.0l
Glucose5.0g
Tryptone or Trypticase10.0g
Yeast extract3.0g
Na2S x 9 H2O1.0g
Vitamin solution (see medium 116)5.0ml
Resazurin1.0mg
FeSO4 x 7 H2O3.0mg
Trace element solution (see below)9.0ml
K2HPO41.5g
KH2PO40.75g
MgCl2 x 6 H2O0.4g
NaCl0.9g
NH4Cl0.9g
The medium is prepared under 100% N2 gas. Glucose is sterilized separately. Final pH of the medium is 7.2 - 7.4.
 
Trace element solution
Distilled water1.0l
Na2SeO3 x 5 H2O0.02g
NaCl1.0g
NiCl2 x 6 H2O0.026g
Na2MoO4 x 2 H2O0.01g
H3BO30.01g
CuCl20.02g
ZnCl20.1g
CaCl2 x 2 H2O0.1g
CoCl2 x 6 H2O0.17g
MnCl2 x 4 H2O0.1g
FeCl2 x 4 H2O0.2g
Nitrilotriacetic acid12.8g
First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH.
 

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