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130 - CLOSTRIDIUM LJUNGDAHLII MEDIUM
Distilled water1.0l
Na2S x 9 H2O0.3g
Cysteine-HCl x H2O0.3g
Fructose5.0g
NaHCO31.0g
Vitamin solution (see medium 116)10.0ml
Na2WO4 x 2 H2O0.2mg
Trace element solution (see medium 116)10.0ml
Yeast extract1.0g
CaCl2 x 2 H2O0.02g
KH2PO40.1g
NaCl0.8g
MgSO4 x 7 H2O0.2g
KCl0.1g
NH4Cl1.0g
Prepare medium anaerobically under 80% N2 + 20% CO2 gas mixture. Add ingredients (except bicarbonate, fructose and reducing agents), boil, cool under N2 + CO2 and dispense anaerobically under same gas. Autoclave at 121°C for 15 min. Add fructose (N2), bicarbonate (N2 + CO2), cysteine (N 2) and sulfide (N2) from sterile, anaerobic stock solutions. Final pH is 5.9.
 

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