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| 130 - CLOSTRIDIUM LJUNGDAHLII MEDIUM | ||
| Distilled water | 1.0 | l |
| Na2S x 9 H2O | 0.3 | g |
| Cysteine-HCl x H2O | 0.3 | g |
| Fructose | 5.0 | g |
| NaHCO3 | 1.0 | g |
| Vitamin solution (see medium 116) | 10.0 | ml |
| Na2WO4 x 2 H2O | 0.2 | mg |
| Trace element solution (see medium 116) | 10.0 | ml |
| Yeast extract | 1.0 | g |
| CaCl2 x 2 H2O | 0.02 | g |
| KH2PO4 | 0.1 | g |
| NaCl | 0.8 | g |
| MgSO4 x 7 H2O | 0.2 | g |
| KCl | 0.1 | g |
| NH4Cl | 1.0 | g |
| Prepare medium anaerobically under 80% N2 + 20% CO2 gas mixture. Add ingredients (except bicarbonate, fructose and reducing agents), boil, cool under N2 + CO2 and dispense anaerobically under same gas. Autoclave at 121°C for 15 min. Add fructose (N2), bicarbonate (N2 + CO2), cysteine (N 2) and sulfide (N2) from sterile, anaerobic stock solutions. Final pH is 5.9. | ||