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> Media 133
133 - ALICYCLOBACILLUS MEDIUM
Sterilize solutions separately. For liquid medium combine solution A (with 1.0 l distilled water) and solution B. For solid medium combine solution A (with 0.5 l distilled water), solution B and solution C.
Solution A
Distilled water (for solid medium)
0.5
l
Distilled water (for liquid medium)
1.0
l
KH
2
PO
4
3.0
g
Glucose
5.0
g
Yeast extract
2.0
g
(NH
4
)
2
SO
4
0.2
g
MgSO
4
x 7 H
2
O
0.5
g
CaCl
2
x 2 H
2
O
0.25
g
Adjust pH to 4.0
Solution B
Trace element sol. SL-6
1.0
ml
Solution C
Distilled water
0.5
l
Agar
15.0
g
Trace element solution SL-6
Distilled water
1.00
l
Na
2
MoO
4
x 2 H
2
O
0.03
g
NiCl
2
x 6 H
2
O
0.02
g
CuCl
2
x 2 H
2
O
0.01
g
CoCl
2
x 6 H
2
O
0.20
g
H
3
BO
3
0.30
g
MnCl
2
x 4 H
2
O
0.03
g
ZnSO
4
x 7 H
2
O
0.10
g
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