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133 - ALICYCLOBACILLUS MEDIUM
Sterilize solutions separately. For liquid medium combine solution A (with 1.0 l distilled water) and solution B. For solid medium combine solution A (with 0.5 l distilled water), solution B and solution C.
 
Solution A
Distilled water (for solid medium)0.5l
Distilled water (for liquid medium)1.0l
KH2PO43.0g
Glucose5.0g
Yeast extract2.0g
(NH4)2SO40.2g
MgSO4 x 7 H2O0.5g
CaCl2 x 2 H2O0.25g
Adjust pH to 4.0
 
Solution B
Trace element sol. SL-61.0ml
 
 
Solution C
Distilled water0.5l
Agar15.0g
 
 
Trace element solution SL-6
Distilled water1.00l
Na2MoO4 x 2 H2O0.03g
NiCl2 x 6 H2O0.02g
CuCl2 x 2 H2O0.01g
CoCl2 x 6 H2O0.20g
H3BO30.30g
MnCl2 x 4 H2O0.03g
ZnSO4 x 7 H2O0.10g
 
 

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