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134 - RAE Medium (Reinforced AE Medium)
Distilled water1.0l
Acetic acid10.0ml
Ethanol20.0ml
Citric acid x H2O1.5g
Na2HPO4 x 2 H2O3.38g
Yeast extract10.0g
Peptone10.0g
Glucose40.0g
The ethanol and acetic acid are sterilized separately by filtration and added aseptically to the sterile medium. A double layer system has to be used. A layer of growth medium containing 1% agar is poured into a plate and allowed to solidify. A thin layer of the growth medium containing 2% agar is then poured on top.
 

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