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135 - MINERAL MEDIUM WITH TOLUENE
Distilled water1.0l
Nitrilotriacetic acid (NTA)10.0mg
CoSO410.0µg
ZnSO4 x 7 H2O0.2mg
MnSO4 x H2O3.0mg
Ferric ammonium citrate5.0mg
MgSO4 x 7 H2O0.2g
NH4Cl2.0g
KH2PO41.5g
Na2HPO4 x 12 H2O9.0g
Adjust pH to 7.0. One drop of toluene is evaporated in a desiccator. 0.1% succinate may be added to the medium as additional carbon source.
 

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