Online catalogue

137 - BASALMEDIUM FOR DEGRADATIVE PSEUDOMONADS
Tap water1.0l
Agar15.0g
NH4-Fe(III)-citrate50.0mg
(NH4)2SO41.0g
CaCl2 x 2 H2O10.0mg
MgSO4 x 7 H2O5.0g
KH2PO40.4g
K2HPO41.6g
Dissolve and autoclave all components separately. Add agar after cooling. Add agar after cooling. Use the respective carbon source, e.g. toluol as vapor or salicylic acid (0.05%) to the basalmedium. Add also 50 µg/ml tryptophan after autoclaving for better growth. Grow plasmid-bearing strains always under selective pressure.
 

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