|
|
|
| 137 - BASALMEDIUM FOR DEGRADATIVE PSEUDOMONADS | ||
| Tap water | 1.0 | l |
| Agar | 15.0 | g |
| NH4-Fe(III)-citrate | 50.0 | mg |
| (NH4)2SO4 | 1.0 | g |
| CaCl2 x 2 H2O | 10.0 | mg |
| MgSO4 x 7 H2O | 5.0 | g |
| KH2PO4 | 0.4 | g |
| K2HPO4 | 1.6 | g |
| Dissolve and autoclave all components separately. Add agar after cooling. Add agar after cooling. Use the respective carbon source, e.g. toluol as vapor or salicylic acid (0.05%) to the basalmedium. Add also 50 µg/ml tryptophan after autoclaving for better growth. Grow plasmid-bearing strains always under selective pressure. | ||