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140 - M63 MEDIUM
Solution C1ml
Solution B20ml
Sterile water779ml
Solution A200ml
Mix solution A with sterile water. Add solutions B and C. Mix well and aseptically prepare plates, tubes or bottles.
 
Solution A
pH 7.0
Distilled water to1.0l
FeSO4 x 7 H2O2.5g
(NH4)2SO410.0g
KH2PO468.0g
Dissolve the components to water by heating. Autoclave 121°C for 15 min.
 
Solution B
Distilled water to200.0ml
Casamino acids50.0g
Dissolve amino acids to water by heating and filter-sterilize.
 
Solution C
Distilled water to100.0ml
MgSO4 x 7 H2O24.65g
Mix carefully and filter-sterilize.
 

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