142 - PYG-MEDIUM (modified)
Cysteine-HCl x H2O0.5g
Vitamin K1 solution (see below)0.20ml
Haemin solution (see below)10.0ml
Distilled water950.0ml
Salt solution (see medium 93)40.0ml
Tween 801.0ml
Beef extract5.0g
Yeast extract10.0g
Trypticase peptone5.0g
The vitamin K1, haemin solution and cysteine are added after the medium has been boiled and cooled under CO2. Adjust pH to 7.2 using 8 N NaOH. Distribute under N2 and autoclave.
Haemin solution
Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water. Store refrigerated.
Vitamin solution
Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle for max. one month.

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