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143 - MODIFIED CHOPPED MEAT MEDIUM
Milli-Q water to final volume1000ml
Vitamin solution (see below)0.2ml
Hemin solution (see below)10.0ml
L-cysteine-HCl0.5g
Agar, if necessary20.0g
Resazurin solution1.0ml
K2HPO45.0g
Yeast extract5.0g
Cooked Meat Medium Oxoid CM 81
Add all other components except of Cooked Meat Medium to distilled water and mix well. Adjust pH to 7.0. Weight Cooked Meat Medium (3 g) directly into Schott bottles (50 ml) and add 30 ml solution above. Check pH from one bottle. Autoclave at 121°C for 15 min and transfer the bottles into an anaerobic cabinet. Store for 1 month in dark at + 4°C
 
Hemin solution
Distilled water to100.0ml
1 N NaOH1.0ml
Hemin50.0mg
Dissolve hemin in NaOH and add distilled water. Autoclave at 121°C for 15 min.
 
Resazurin solution
Distilled water50.0ml
Resazurin0.05g
Dissolve resazurin in distilled water.
 
Vitamin solution
95% ethanol20.0ml
Vitamin K10.1g
Dissolve vitamin in ethanol and filter sterilize. Store solution in brown bottle under refrigeration. Discard after one month.
 

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