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| 143 - MODIFIED CHOPPED MEAT MEDIUM | ||
| Milli-Q water to final volume | 1000 | ml |
| Vitamin solution (see below) | 0.2 | ml |
| Hemin solution (see below) | 10.0 | ml |
| L-cysteine-HCl | 0.5 | g |
| Agar, if necessary | 20.0 | g |
| Resazurin solution | 1.0 | ml |
| K2HPO4 | 5.0 | g |
| Yeast extract | 5.0 | g |
| Cooked Meat Medium Oxoid CM 81 | ||
| Add all other components except of Cooked Meat Medium to distilled water and mix well. Adjust pH to 7.0. Weight Cooked Meat Medium (3 g) directly into Schott bottles (50 ml) and add 30 ml solution above. Check pH from one bottle. Autoclave at 121°C for 15 min and transfer the bottles into an anaerobic cabinet. Store for 1 month in dark at + 4°C | ||
| Hemin solution | ||
| Distilled water to | 100.0 | ml |
| 1 N NaOH | 1.0 | ml |
| Hemin | 50.0 | mg |
| Dissolve hemin in NaOH and add distilled water. Autoclave at 121°C for 15 min. | ||
| Resazurin solution | ||
| Distilled water | 50.0 | ml |
| Resazurin | 0.05 | g |
| Dissolve resazurin in distilled water. | ||
| Vitamin solution | ||
| 95% ethanol | 20.0 | ml |
| Vitamin K1 | 0.1 | g |
| Dissolve vitamin in ethanol and filter sterilize. Store solution in brown bottle under refrigeration. Discard after one month. | ||