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148 - R Medium
Distilled water916.00ml
Trace element solution1.00ml
Solution 41.00ml
Solution 310.00ml
Solution 20.20ml
Solution 15.00ml
Combine the KH2PO4 and the distilled water adjusting the pH to 6.8 using NaOH. Autoclave at 121ºC for 15 minutes and cool the medium to 56ºC. Filter sterilize solutions 1-4 and the trace element solution. Aseptically add them one at a time to the autoclaved solution.
Solution 1
Distilled water200.00ml
FeSO4 x 7 H2O0.40g
MgSO4 x 7 H2O16.00g
Solution 2
Distilled water10.00ml
Solution 3
Distilled water10.00ml
Solution 4
Solution 4 may not go completely into solution. It may be necessary to shake or sonicate to break-up the clumps. The final concentration needs to be between 100-500 µg/ml. Shake well and filter sterilize.
Trace element solution
Distilled water1.00l
NiCl2 x 6 H2O2.00mg
Na2MoO4 x 2 H2O3.00mg
CuCl2 x 2 H2O1.00mg
CoCl2 x 6 H2O20.00mg
MnCl2 x 4 H2O3.00mg
ZnSO4 x 7 H2O10.00mg

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