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160 - GEOBACTER MEDIUM
Distilled water980.00ml
NaHCO32.5g
Na2-fumarate8.00g
Resazurin0.50mg
Selenite-tungstate solution1.00ml
Vitamin solution10.00ml
Trace element solution10.00ml
Na-acetate0.82g
KCl0.10g
Na2HPO40.60g
NH4Cl1.50g
Dissolve ingredients (except fumarate and NaHCO3), bring to a boil, cool down to room temperature while gasing the medium with 80% N2 + 20% CO2, and add the bicarbonat. Equilibrate the medium with gas to reach a pH of 6.8. Dispense the medium in anaerobe vials under N2 + CO2. After autoclaving add fumarate from an anaerobic filter sterilized stock solution (e.g. 16% w/v Na2-fumarate; add 0.5 ml/10 ml medium). Check medium pH which should be around 6.8.
 
Trace element solution
Distilled water1000.00ml
Na2SeO3 x 5 H2O0.30mg
NiCl2 x 6 H2O0.03g
Na2MoO4 x 2 H2O0.01g
H3BO30.01g
KAl(SO4)2 x 12 H2O0.02g
CuSO4 x 5 H2O0.01g
ZnSO4 x 7 H2O0.18g
CaCl2 x 2 H2O0.10g
CoSO4 x 7 H2O0.18g
FeSO4 x 7 H2O0.10g
NaCl1.00g
MnSO4 x H2O0.50g
MgSO4 x 7 H2O3.00g
Nitriloacetic acid1.50g
First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH).
 
Vitamin solution
Distilled water1000.00ml
Lipoic acid5.00mg
p-Aminobenzoic acid5.00mg
Vitamin B120.10mg
D-Ca-pantothenate5.00mg
Nicotinic acid5.00mg
Riboflavin5.00mg
Thiamine-HCl x 2 H2O5.00mg
Pyridoxine-HCl10.00mg
Folic acid2.00mg
Biotin2.00mg
 
 

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