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166 - NITROSOMONAS EUROPAEA MEDIUM
Combine solutions 1, 2 and 3. Dispense aseptically into desired aliquots.
 
Solution 1
(NH4)2SO4 (for 50 mM NH4)4.95g
KH2PO40.62g
MgSO4 x 7 H2O0.27g
CaCl2 x 2 H2O0.04g
FeSO4 (30 mM in 50 mM EDTA at pH 7.0)0.5ml
CuSO4 x 5 H2O0.2mg
Distilled water1.2L
Filter sterilize.
 
Solution 2
KH2PO48.2g
NaH2PO40.7g
Distilled water300.0ml
Bring to pH 8.0 with 10 N NaOH. Filter sterilize.
 
Solution 3
Na2CO3 anhydrous0.6g
Distilled water12.0ml
Filter sterilize.
 

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