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| 166 - NITROSOMONAS EUROPAEA MEDIUM | ||
| Combine solutions 1, 2 and 3. Dispense aseptically into desired aliquots. | ||
| Solution 1 | ||
| (NH4)2SO4 (for 50 mM NH4) | 4.95 | g |
| KH2PO4 | 0.62 | g |
| MgSO4 x 7 H2O | 0.27 | g |
| CaCl2 x 2 H2O | 0.04 | g |
| FeSO4 (30 mM in 50 mM EDTA at pH 7.0) | 0.5 | ml |
| CuSO4 x 5 H2O | 0.2 | mg |
| Distilled water | 1.2 | L |
| Filter sterilize. | ||
| Solution 2 | ||
| KH2PO4 | 8.2 | g |
| NaH2PO4 | 0.7 | g |
| Distilled water | 300.0 | ml |
| Bring to pH 8.0 with 10 N NaOH. Filter sterilize. | ||
| Solution 3 | ||
| Na2CO3 anhydrous | 0.6 | g |
| Distilled water | 12.0 | ml |
| Filter sterilize. | ||