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168 - THIOALKALIVIBRIO SULFIDIPHILUS MEDIUM
Basic medium (pH 10, 0.6 M total Na+)
NaHCO38g
Na2CO322g
NaCl6g
K2HPO41g
Distilled water1000ml
Sterilize in Schott closed bottles 20 min at 120 C. Age for minimum 3 days to allow precipitate formation on the bottom. Carefully decant clear solution to another sterile bottle.
 
After sterilization:
4 mM NH4Cl from 2 M stock
40 mM sodium thiosulfate from 2 M stock
1 ml/L trace metals (Pfennig&Lippert, 1966)
1 mM MgCl2 x 6H2O
White cloudy presipitate forming after Mg addition can be dissolved by vigorous mixing.
 

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