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169 - Nitrate mineral salts medium (NMS)
MgSO4 x 7 H2O1.0g
CaCl2 x 6 H2O0.20g
Chelated Iron Solution2.0ml
KNO31.0g
Trace Element Solution0.5ml
KH2PO40.272g
Na2HPO4 x 12 H2O0.717g
Agar12.5g
Distilled deionized water1.0L
Adjust pH to 6.8. Autoclave at 121 C for 15 minutes.
 
Chelated Iron Solution
Ferric (III) ammonium citrate*0.1g
EDTA, sodium salt0.2g
HCL concentrated0.3ml
Distilled deionized water100.0ml
* 0.05 g of Ferric (III) chloride may be substituted. Use 2.0 ml of this chelated iron solution per liter of final medium.
 
Trace Element Solution
EDTA500.0mg
FeSO4 x 7 H2O200.0mg
ZnSO4 x 7 H2O10.0mg
MnCl2 x 4 H2O3.0mg
H3BO330.0mg
CoCl2 x 6 H2O20.0mg
CaCl2 x 2 H2O1.0mg
NiCl2 x 6 H2O2.0mg
Na2MoO4 x 2 H2O3.0mg
Distilled water1.0L
Autoclave at 121 C for 15 minutes.
 

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