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Media
> Media 169
169 - Nitrate mineral salts medium (NMS)
MgSO
4
x 7 H
2
O
1.0
g
CaCl
2
x 6 H
2
O
0.20
g
Chelated Iron Solution
2.0
ml
KNO
3
1.0
g
Trace Element Solution
0.5
ml
KH
2
PO
4
0.272
g
Na
2
HPO
4
x 12 H
2
O
0.717
g
Agar
12.5
g
Distilled deionized water
1.0
L
Adjust pH to 6.8. Autoclave at 121 C for 15 minutes.
Chelated Iron Solution
Ferric (III) ammonium citrate*
0.1
g
EDTA, sodium salt
0.2
g
HCL concentrated
0.3
ml
Distilled deionized water
100.0
ml
* 0.05 g of Ferric (III) chloride may be substituted. Use 2.0 ml of this chelated iron solution per liter of final medium.
Trace Element Solution
EDTA
500.0
mg
FeSO
4
x 7 H
2
O
200.0
mg
ZnSO
4
x 7 H
2
O
10.0
mg
MnCl
2
x 4 H
2
O
3.0
mg
H
3
BO
3
30.0
mg
CoCl
2
x 6 H
2
O
20.0
mg
CaCl
2
x 2 H
2
O
1.0
mg
NiCl
2
x 6 H
2
O
2.0
mg
Na
2
MoO
4
x 2 H
2
O
3.0
mg
Distilled water
1.0
L
Autoclave at 121 C for 15 minutes.
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