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| 169 - Nitrate mineral salts medium (NMS) | ||
| MgSO4 x 7 H2O | 1.0 | g |
| CaCl2 x 6 H2O | 0.20 | g |
| Chelated Iron Solution | 2.0 | ml |
| KNO3 | 1.0 | g |
| Trace Element Solution | 0.5 | ml |
| KH2PO4 | 0.272 | g |
| Na2HPO4 x 12 H2O | 0.717 | g |
| Agar | 12.5 | g |
| Distilled deionized water | 1.0 | L |
| Adjust pH to 6.8. Autoclave at 121 C for 15 minutes. | ||
| Chelated Iron Solution | ||
| Ferric (III) ammonium citrate* | 0.1 | g |
| EDTA, sodium salt | 0.2 | g |
| HCL concentrated | 0.3 | ml |
| Distilled deionized water | 100.0 | ml |
| * 0.05 g of Ferric (III) chloride may be substituted. Use 2.0 ml of this chelated iron solution per liter of final medium. | ||
| Trace Element Solution | ||
| EDTA | 500.0 | mg |
| FeSO4 x 7 H2O | 200.0 | mg |
| ZnSO4 x 7 H2O | 10.0 | mg |
| MnCl2 x 4 H2O | 3.0 | mg |
| H3BO3 | 30.0 | mg |
| CoCl2 x 6 H2O | 20.0 | mg |
| CaCl2 x 2 H2O | 1.0 | mg |
| NiCl2 x 6 H2O | 2.0 | mg |
| Na2MoO4 x 2 H2O | 3.0 | mg |
| Distilled water | 1.0 | L |
| Autoclave at 121 C for 15 minutes. | ||