Online catalogue

172 - EMM Edinburgh minimal medium
EMM 50 x salt stock (for 1 litre filter sterilize)
MgCl2 x 6 H2O52.5g
CaCl2 x 2 H2O0.735g
KCl50g
Na2SO42g
 
 
EMM 10000x mineral stock (for 1 litre filter sterilize)
H3BO45g
MnSO44g
ZnSO4 x 7 H2O4g
FeCl3 x 6 H2O2g
H2MoO4 (molybdic acid)2g
KI1g
CuSo4 x 5 H2O0.4g
Citric acid10g
 
 
EMM 1000x vitamin stock (for 1 litre filter sterilize)
Panthothenic acid1g
Nicotinic acid10g
Inositol10g
Biotin10mg
 
 
EMM 5mM thiamine stock (for 100 ml filter sterilize)
Thiamine0.168g
 
 
EMM plates and expression medium (for 1 litre)
Ammonium chloride5g
Potassium hydrogen phthalate3g
Disodiumhydrogen phosphate dihydrat (Na2HPO4 x 2 H2O2.2g
Glucose20g
Adjust pH between 5.4 to5.8.
 
EMM plates
Agar40g
EMM 50 x salt stock solution20ml
EMM 10000 x mineral stock solution0.1ml
Autoclave. At 55-60 C add 1 ml of EMM 1000 x vitamin stock and EMM 5 mM thiamine stock.
 
EMM expression medium (for 1 litre)
EMM 50 x salt stock solution20ml
EMM 10000 x mineral stock solution0.1ml
Autoclave. Add 1 ml of EMM 1000 x vitamin stock.
 

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