Online catalogue
Strain search
Brewer's yeasts
Prices
Ordering
Nomenclature
Patent deposits
Contact
Collections
Bacteria
Filamentous fungi
Baker's yeasts
Brewer's yeasts
Other yeasts
Strain search
Media
References
Abbreviations
Online catalogue
Catalogue
>
Media
> Media 172
172 - EMM Edinburgh minimal medium
EMM 50 x salt stock (for 1 litre filter sterilize)
MgCl
2
x 6 H
2
O
52.5
g
CaCl
2
x 2 H
2
O
0.735
g
KCl
50
g
Na
2
SO
4
2
g
EMM 10000x mineral stock (for 1 litre filter sterilize)
H
3
BO
4
5
g
MnSO
4
4
g
ZnSO
4
x 7 H
2
O
4
g
FeCl
3
x 6 H
2
O
2
g
H
2
MoO
4
(molybdic acid)
2
g
KI
1
g
CuSo
4
x 5 H
2
O
0.4
g
Citric acid
10
g
EMM 1000x vitamin stock (for 1 litre filter sterilize)
Panthothenic acid
1
g
Nicotinic acid
10
g
Inositol
10
g
Biotin
10
mg
EMM 5mM thiamine stock (for 100 ml filter sterilize)
Thiamine
0.168
g
EMM plates and expression medium (for 1 litre)
Ammonium chloride
5
g
Potassium hydrogen phthalate
3
g
Disodiumhydrogen phosphate dihydrat (Na
2
HPO
4
x 2 H
2
O
2.2
g
Glucose
20
g
Adjust pH between 5.4 to5.8.
EMM plates
Agar
40
g
EMM 50 x salt stock solution
20
ml
EMM 10000 x mineral stock solution
0.1
ml
Autoclave. At 55-60 C add 1 ml of EMM 1000 x vitamin stock and EMM 5 mM thiamine stock.
EMM expression medium (for 1 litre)
EMM 50 x salt stock solution
20
ml
EMM 10000 x mineral stock solution
0.1
ml
Autoclave. Add 1 ml of EMM 1000 x vitamin stock.
Printable version