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| 172 - EMM Edinburgh minimal medium | ||
| EMM 50 x salt stock (for 1 litre filter sterilize) | ||
| MgCl2 x 6 H2O | 52.5 | g |
| CaCl2 x 2 H2O | 0.735 | g |
| KCl | 50 | g |
| Na2SO4 | 2 | g |
| EMM 10000x mineral stock (for 1 litre filter sterilize) | ||
| H3BO4 | 5 | g |
| MnSO4 | 4 | g |
| ZnSO4 x 7 H2O | 4 | g |
| FeCl3 x 6 H2O | 2 | g |
| H2MoO4 (molybdic acid) | 2 | g |
| KI | 1 | g |
| CuSo4 x 5 H2O | 0.4 | g |
| Citric acid | 10 | g |
| EMM 1000x vitamin stock (for 1 litre filter sterilize) | ||
| Panthothenic acid | 1 | g |
| Nicotinic acid | 10 | g |
| Inositol | 10 | g |
| Biotin | 10 | mg |
| EMM 5mM thiamine stock (for 100 ml filter sterilize) | ||
| Thiamine | 0.168 | g |
| EMM plates and expression medium (for 1 litre) | ||
| Ammonium chloride | 5 | g |
| Potassium hydrogen phthalate | 3 | g |
| Disodiumhydrogen phosphate dihydrat (Na2HPO4 x 2 H2O | 2.2 | g |
| Glucose | 20 | g |
| Adjust pH between 5.4 to5.8. | ||
| EMM plates | ||
| Agar | 40 | g |
| EMM 50 x salt stock solution | 20 | ml |
| EMM 10000 x mineral stock solution | 0.1 | ml |
| Autoclave. At 55-60 C add 1 ml of EMM 1000 x vitamin stock and EMM 5 mM thiamine stock. | ||
| EMM expression medium (for 1 litre) | ||
| EMM 50 x salt stock solution | 20 | ml |
| EMM 10000 x mineral stock solution | 0.1 | ml |
| Autoclave. Add 1 ml of EMM 1000 x vitamin stock. | ||