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173 - BACILLUS ACIDOCALDARIUS MEDIUM
Solution A
Yeast extract1.00g
(NH4)2SO40.20g
MgSO4 x 7 H2O0.50g
CaCl2 x 2 H2O0.25g
KH2PO40.60g
Distilled water500.00ml
Adjust pH to 3.0 - 4.0.
 
Solution B
Glucose1.00g
Agar20.00g
Distilled water500.00ml
Sterilize solution A and B separately at 121 C for 15 min. Cool to 50 C and then combine.
 

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