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173 - BACILLUS ACIDOCALDARIUS MEDIUM | ||
Solution A | ||
Yeast extract | 1.00 | g |
(NH4)2SO4 | 0.20 | g |
MgSO4 x 7 H2O | 0.50 | g |
CaCl2 x 2 H2O | 0.25 | g |
KH2PO4 | 0.60 | g |
Distilled water | 500.00 | ml |
Adjust pH to 3.0 - 4.0. | ||
Solution B | ||
Glucose | 1.00 | g |
Agar | 20.00 | g |
Distilled water | 500.00 | ml |
Sterilize solution A and B separately at 121 C for 15 min. Cool to 50 C and then combine. | ||