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Media
> Media 176
176 - LEPTOSPIRILLUM (HH) MEDIUM
Solution A
(NH
4
)
2
SO
4
132.0
mg
MgCl
2
x 6 H
2
O
53.0
mg
KH
2
PO
4
27.0
mg
CaCl
2
x 2 H
2
O
147.0
mg
Distilled water
950.0
ml
Adjust pH to 1.8 with 10N sulphuric acid.
Solution B
FeSO
4
x 7 H
2
O
20.0
g
H
2
SO
4
0,25 N
50.0
ml
The pH of this solution should be 1.2.
Trace element solution:
MnCl
2
x 2 H
2
O
62.0
mg
ZnCl
2
68.0
mg
CoCl
2
x 6 H
2
O
64.0
mg
H
3
BO
3
31.0
mg
Na
2
MoO
4
10.0
mg
CuCl
2
x 2 H
2
O
67.0
mg
Distilled water
1000.0
ml
Adjust pH to 1.8 with 10N sulphuric acid.
Autoclave the solutions separately at 112ºC for 30 min. Prior to use mix solutions A and B and add 1 ml of Trace element solution. The final pH of the medium should be 1.8.
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