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176 - LEPTOSPIRILLUM (HH) MEDIUM
Solution A
(NH4)2SO4132.0mg
MgCl2 x 6 H2O53.0mg
KH2PO427.0mg
CaCl2 x 2 H2O147.0mg
Distilled water950.0ml
Adjust pH to 1.8 with 10N sulphuric acid.
 
Solution B
FeSO4 x 7 H2O20.0g
H2SO4 0,25 N50.0ml
The pH of this solution should be 1.2.
 
Trace element solution:
MnCl2 x 2 H2O62.0mg
ZnCl268.0mg
CoCl2 x 6 H2O64.0mg
H3BO331.0mg
Na2MoO410.0mg
CuCl2 x 2 H2O67.0mg
Distilled water1000.0ml
Adjust pH to 1.8 with 10N sulphuric acid.
 
Autoclave the solutions separately at 112ºC for 30 min. Prior to use mix solutions A and B and add 1 ml of Trace element solution. The final pH of the medium should be 1.8.
 

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