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177 - Mineral medium for chemolithotrophic growth (H-3)
Solution A
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KH2PO42.3g
Na2HPO4x 2 H2O2.9g
NH4Cl1.0g
MgSO4 x 7 H2O0.5g
NaHCO30.5g
CaCl2 x 2 H2O0.01g
Trace element solution SL-65.0ml
Distilled water980.0ml
Agar (if necessary)15.00g
Adjust pH to 6.8. Autoclave for 15 min at 121 C. Add filter sterilised Fe(NH4) citrate to the medium.
 
Trace element solution SL-6
ZnSO4 x 7 H2O0.10g
MnCl2 x 4 H2O0.03g
H3BO30.30g
CoCl2 x 6 H2O0.20g
CuCl2 x 2 H2O0.01g
NiCl2 x 6 H2O0.02g
Na2Mo4 x 2 H2O0.03g
Distilled water1000ml
 
 
Fe(NH4) citrate solution
Fe(NH4)citrate0.05g
Distilled water20ml
Filter sterilise.
 

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