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180 - Acidithiobacillus ferrooxidans medium (APH)
(NH4)2SO42.00g
K2HPO40.50g
MgSO4 x 7 H2O0.50g
KCl0.10g
Ca(NO3)20.01g
FeSO4 x 7 H2O8.00g
Distilled water1000.00ml
Dissolve ingredients and adjust pH to 2.0 with 10 N H2SO4. Sterilize the medium by filtration. Alternatively, autoclave separately the basal medium (pH adjusted to 2.0) and the ferrous sulfate (8.00 g FeSO4 x 7 H2O in 50 ml distilled water, pH not adjusted, in a sealed vessel under nitrogen gas atmosphere). VTT E-991376: Omit the ferrous sulfate from the medium and add 10.00 g / L elemental sulfur as substrate. For sterilization place the sulfur in screw-capped tubes, add 1-2 drops of distilled water and incubate on 3 successive days for 3 h at 90-100°C in a water bath. Before use, aseptically layer the sulfur onto the surface of autoclaved liquid basal medium, Adjust pH of completed medium to 2.0.
 

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