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| 180 - Acidithiobacillus ferrooxidans medium (APH) | ||
| (NH4)2SO4 | 2.00 | g |
| K2HPO4 | 0.50 | g |
| MgSO4 x 7 H2O | 0.50 | g |
| KCl | 0.10 | g |
| Ca(NO3)2 | 0.01 | g |
| FeSO4 x 7 H2O | 8.00 | g |
| Distilled water | 1000.00 | ml |
| Dissolve ingredients and adjust pH to 2.0 with 10 N H2SO4. Sterilize the medium by filtration. Alternatively, autoclave separately the basal medium (pH adjusted to 2.0) and the ferrous sulfate (8.00 g FeSO4 x 7 H2O in 50 ml distilled water, pH not adjusted, in a sealed vessel under nitrogen gas atmosphere). VTT E-991376: Omit the ferrous sulfate from the medium and add 10.00 g / L elemental sulfur as substrate. For sterilization place the sulfur in screw-capped tubes, add 1-2 drops of distilled water and incubate on 3 successive days for 3 h at 90-100°C in a water bath. Before use, aseptically layer the sulfur onto the surface of autoclaved liquid basal medium, Adjust pH of completed medium to 2.0. | ||