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184 - Modified Reinforced Clostridial medium
Agar medium
Reinforced Clostridial Medium (BD 218081)38g
Agar14,5g
Distilled water1000ml
 
 
Broth medium
Peptone10.0g
Beef extract10.0g
Yeast extract3.0g
Dextrose5.0g
NaCl5.0g
Soluble starch1.0g
L-Cysteine HCl0.5g
Sodium acetate3.0g
Resazurin (0.025%)4.0ml
Distilled water1000ml
Combine ingredients and dissolve. Adjust pH to 6.8. Dispense and autoclave at 121°C. BD and Oxoid Reinforced Clostridial formulations contain agar. This modification is agar free.
 

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