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| 184 - Modified Reinforced Clostridial medium | ||
| Agar medium | ||
| Reinforced Clostridial Medium (BD 218081) | 38 | g |
| Agar | 14,5 | g |
| Distilled water | 1000 | ml |
| Broth medium | ||
| Peptone | 10.0 | g |
| Beef extract | 10.0 | g |
| Yeast extract | 3.0 | g |
| Dextrose | 5.0 | g |
| NaCl | 5.0 | g |
| Soluble starch | 1.0 | g |
| L-Cysteine HCl | 0.5 | g |
| Sodium acetate | 3.0 | g |
| Resazurin (0.025%) | 4.0 | ml |
| Distilled water | 1000 | ml |
| Combine ingredients and dissolve. Adjust pH to 6.8. Dispense and autoclave at 121°C. BD and Oxoid Reinforced Clostridial formulations contain agar. This modification is agar free. | ||