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185 - OM-2 Medium
NH4-oxalate15.00g
NaHCO310.00g
NaH2PO4 x 2 H2O10.00g
NaCl0.70g
KCl0.57g
MgCl2 x 6 H2O0.10g
CaCl2 x 2 H2O0.01g
Na2S2O3 x 5 H2O1.00g
Distilled water1000ml
pH 6.8-7.0
The first three salts must be dissolved in the indicated sequence. After autoclaving for 20 min at 121°C the pH of the medium shifted to 8.5-9.0. Solid medium contained 1.5 to 2.0% (w/v) of Bacto-Agar (Difco).
 

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