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186 - Sulfobacillus Medium
Solution A
(NH4)2SO43.00g
KCl0.10g
K2HPO40.50g
MgSO4 x 7 H2O0.50g
Ca(NO3)20.01g
Distilled water700ml
Adjust pH to 2.0-2.2 with sulfuric acid.
 
Solution B
FeSO4 x 7 H2O44.20g
Distilled water300ml
H2SO4, 10 N1.00ml
Sterilize by filtration or handle under N2 before autoclaving to prevent oxidation of the iron.
 
Solution C
Yeast extract (1% w/v in water)20ml
After autoclaving, combine the three solutions. Medium pH 1.9-2.4.
 

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