186 - Sulfobacillus Medium
Solution A
MgSO4 x 7 H2O0.50g
Distilled water700ml
Adjust pH to 2.0-2.2 with sulfuric acid.
Solution B
FeSO4 x 7 H2O44.20g
Distilled water300ml
H2SO4, 10 N1.00ml
Sterilize by filtration or handle under N2 before autoclaving to prevent oxidation of the iron.
Solution C
Yeast extract (1% w/v in water)20ml
After autoclaving, combine the three solutions. Medium pH 1.9-2.4.

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