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| 186 - Sulfobacillus Medium | ||
| Solution A | ||
| (NH4)2SO4 | 3.00 | g |
| KCl | 0.10 | g |
| K2HPO4 | 0.50 | g |
| MgSO4 x 7 H2O | 0.50 | g |
| Ca(NO3)2 | 0.01 | g |
| Distilled water | 700 | ml |
| Adjust pH to 2.0-2.2 with sulfuric acid. | ||
| Solution B | ||
| FeSO4 x 7 H2O | 44.20 | g |
| Distilled water | 300 | ml |
| H2SO4, 10 N | 1.00 | ml |
| Sterilize by filtration or handle under N2 before autoclaving to prevent oxidation of the iron. | ||
| Solution C | ||
| Yeast extract (1% w/v in water) | 20 | ml |
| After autoclaving, combine the three solutions. Medium pH 1.9-2.4. | ||