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188 - Acidihalobacter prosperus Medium
MgCl2 x 6 H2O2.75g
MgSO4 x 7 H2O3.45g
CaCl2 x 2 H2O0.14g
NiCl2 x 6 H2O solution (0.1 % w/v)2.00ml
Trace element solution10.00ml
Distilled water1000.00ml
Ferrous sulfate solution added prior to inoculation to the final concentration of 2 % (w/v)
Dissolve ingredients, adjust pH to 2.5 with H2SO4 and autoclave. Prior to inoculation add ferrous sulfate from a freshly prepared 40 % (w/v) stock solution.
Ferrous sulfate solution
Prepare a 40 % (w/v) stock solution of FeSO4 x 7 H2O in 0.1 N H2SO4. Sterilize by filtration and store under 100 % N2 gas atmosphere.
Trace element solution
Nitrilotriacetic acid1.50g
MgSO4 x 7 H2O3.00g
MnSO4 x H2O0.50g
FeSO4 x 7 H2O0.10g
CoSO4 x 7 H2O0.18g
CaCl2 x 2 H2O0.10g
ZnSO4 x 7 H2O0.18g
CuSO4 x 5 H2O0.01g
KAl(SO4)2 x 12 H2O0.02g
Na2MoO4 x 2 H2O0.01g
NiCl2 x 6 H2O0.03g
Na2SeO3 x 5 H2O0.30mg
Na2WO4 x 2 H2O0.40mg
Distilled water1000.00ml
First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final pH to 7.0 with KOH.

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