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Filamentous fungi

Brewer's yeasts

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Catalogue > Media > Media 188

188 - Acidihalobacter prosperus Medium
KCl	0.33	g
MgCl₂ x 6 H₂O	2.75	g
MgSO₄ x 7 H₂O	3.45	g
NH₄Cl	1.25	g
CaCl₂ x 2 H₂O	0.14	g
K₂HPO₄	0.14	g
KH₂PO₄	0.14	g
NaCl	0.50	g
NiCl₂ x 6 H₂O solution (0.1 % w/v)	2.00	ml
Trace element solution	10.00	ml
Distilled water	1000.00	ml
Ferrous sulfate solution added prior to inoculation to the final concentration of 2 % (w/v)
Dissolve ingredients, adjust pH to 2.5 with H₂SO₄ and autoclave. Prior to inoculation add ferrous sulfate from a freshly prepared 40 % (w/v) stock solution.

Ferrous sulfate solution

Prepare a 40 % (w/v) stock solution of FeSO₄ x 7 H₂O in 0.1 N H₂SO₄. Sterilize by filtration and store under 100 % N₂ gas atmosphere.

Trace element solution
Nitrilotriacetic acid	1.50	g
MgSO₄ x 7 H₂O	3.00	g
MnSO₄ x H₂O	0.50	g
NaCl	1.00	g
FeSO₄ x 7 H₂O	0.10	g
CoSO₄ x 7 H₂O	0.18	g
CaCl₂ x 2 H₂O	0.10	g
ZnSO₄ x 7 H₂O	0.18	g
CuSO₄ x 5 H₂O	0.01	g
KAl(SO₄)₂ x 12 H₂O	0.02	g
H₃BO₃	0.01	g
Na₂MoO₄ x 2 H₂O	0.01	g
NiCl₂ x 6 H₂O	0.03	g
Na₂SeO₃ x 5 H₂O	0.30	mg
Na₂WO₄ x 2 H₂O	0.40	mg
Distilled water	1000.00	ml
First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final pH to 7.0 with KOH.

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