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189 - Postgate B Medium (Handbook of Microbiological Media, Atlas & Parks, 2000)
Solution A
50 % Sodium lactate solution (GPR, BDH 30746)7.0g
MgSO4 x 7 H2O2.0g
NH4Cl1.0g
CaSO4 x 2 H2O1.2g
Yeast extract1.0g
KH2PO40.5g
Water980ml
Adjust pH to 7.5 and autoclave at 121 °C for 15 minutes. Close the caps and move the bottles to anaerobic atmosphere immediately after autoclaving.
 
Solution B
FeSO4 x 7 H2O0.5g
Water10ml
Autoclave at 121 °C for 15 minutes. Close the caps and move the bottles to anaerobic atmosphere immediately after autoclaving. Add 10 ml of Solution B to Solution A just before use.
 
Solution C
Ascorbic acid C8H8O60.15g
Sodium thioglycolate0.15g
Water15ml
Sterilize by filtration and let prereduce in anaerobic atmosphere. Add 10 ml of Solution C to Solution A just before use.
 
Notes
Solutions A and B can be stored at 4 °C in anaerobic atmosphere for 5 days. Solution C can be stored at 4 °C in anaerobic atmosphere for 2 days.
 

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