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Media
> Media 27
27 - CLOSTRIDIUM THERMOCELLUM MEDIUM
Distilled water
1.0
l
Resazurin
1.0
mg
FeSO
4
× 7 H
2
O
1.1
mg
Na-ß-glycerophosphate
6.0
g
CaCl
2
× 2 H
2
O
0.13
g
K
2
HPO
4
× 3 H
2
O
7.2
g
KH
2
PO
4
1.43
g
MgCl
2
× 6 H
2
O
2.6
g
(NH
4
)
2
SO
4
1.3
g
Glutathione
0.25
g
or Cellobiose
5.0
g
Cellulose (Avicel or MN 300)
10.0
g
Yeast extract
4.5
g
Cellobiose is filter-sterilized separately as a 10 % (w/v) solution in water.
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