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30 - TYX/TYG MEDIUM
*Trace element solution
Distilled water to100.0ml
MgSO41.2g
MnSO4 × 7 H2O0.17g
ZnSO4 × 7 H2O29.0mg
CuSO4 × 5 H2O25.0mg
H2SO42.8ml
FeCl3 × 6 H2O2.7g
CaCl2 × 2 H2O0.15g
CoCl2 × 6 H2O24.0mg
NaMoO4 × 2 H2O0.24g
 
 
Distilled water to1.0l
Resazurin (25 mg/100 ml H2O)4.0ml
Trace element solution*1.0ml
K2HPO43.5g
Na2SO40.2g
Yeast extract5.0g
Tryptone1.0g
Cook 5-10 min (colour turns first red and then yellow). Cool on ice. Add biotin 10.0 mg, p-aminobenzoic acid 10.0 mg and L-cysteinium chloride (monohydr.) 0.5 g. Adjust pH to 7.0. Heat slightly to dissolve (do not boil). Distribute into tubes á 5 ml. After autoclaving (at 121°C for 15 min) add 5 ml sterile sugar solution (10 g/l xylose to TYX and glucose to TYG).
 

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