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| 30 - TYX/TYG MEDIUM | ||
| *Trace element solution | ||
| Distilled water to | 100.0 | ml |
| MgSO4 | 1.2 | g |
| MnSO4 × 7 H2O | 0.17 | g |
| ZnSO4 × 7 H2O | 29.0 | mg |
| CuSO4 × 5 H2O | 25.0 | mg |
| H2SO4 | 2.8 | ml |
| FeCl3 × 6 H2O | 2.7 | g |
| CaCl2 × 2 H2O | 0.15 | g |
| CoCl2 × 6 H2O | 24.0 | mg |
| NaMoO4 × 2 H2O | 0.24 | g |
| Distilled water to | 1.0 | l |
| Resazurin (25 mg/100 ml H2O) | 4.0 | ml |
| Trace element solution* | 1.0 | ml |
| K2HPO4 | 3.5 | g |
| Na2SO4 | 0.2 | g |
| Yeast extract | 5.0 | g |
| Tryptone | 1.0 | g |
| Cook 5-10 min (colour turns first red and then yellow). Cool on ice. Add biotin 10.0 mg, p-aminobenzoic acid 10.0 mg and L-cysteinium chloride (monohydr.) 0.5 g. Adjust pH to 7.0. Heat slightly to dissolve (do not boil). Distribute into tubes á 5 ml. After autoclaving (at 121°C for 15 min) add 5 ml sterile sugar solution (10 g/l xylose to TYX and glucose to TYG). | ||